Room 500 features another meeting with Spooky and a second password plate. Spooky's gift to the player is "fixing" the door, which temporarily sends the player back to room 50. Nothing is new up until the point in room 60, where the player finds the "express" note on the table. However, instead of releasing Specimen 2, reading the note creates a door to the Express Tunnel, which will send the player to Room 512. It should be noted that enemies can spawn in the short walk from room 50-60.
It is possible to start the game from room 500 by inputting the password "CHEESE" in the settings menu.
These rooms add new forest layout rooms, with violent deer-like enemies that attack the player when they get close enough. The player then finds and equips an axe in room 554, embedded into a tree. The axe can kill the deer, but the player will likely take damage when attempting to do so. Interactions with the deer also occasionally display strange messages. There are a few cabin rooms with notes about the deer. In Room 557, there are several doors the player can open. Going through the right door leads to a room with a record player that can be turned on, which causes W.A.M (Wolfgang Amadeus Mozart) to appear. In the left room, the player can find a key to open the middle door. Going through the middle door causes the player to enter Room 558, where they have to use the axe to break the boards nailed to the door. Once the player reach Room 559, Specimen 8 appears in the front hallway and begins to chase them.